Transfected HepG2 Cells for Evaluation of Catechin Effects on Alcohol-Induced CYP2El Cytotoxicity

To evaluate the toxicological properties of human cytochrome p450 2El (CYP2El) induced by ethanol and possible protective effects of various green tea catechins on alcohol-induced toxicity, transfected HepG2 cells that stably and constitutively express human CYP2El were established using the recombi...

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Veröffentlicht in:	Journal of microbiology and biotechnology 2005-12, Vol.15 (6)
Hauptverfasser:	Lee, Y.H. (Korea University, Seoul, Republic of Korea), Ho, J.N. (Korea University, Seoul, Republic of Korea), Dong, M.S. (Korea University, Seoul, Republic of Korea), Park, C.H. (Hanyang University, Seoul, Republic of Korea), Kim, H.K. (Hanseo University, Seosan, Republic of Korea), Hong, B.S. (Korea University, Seoul, Republic of Korea), Shin, D.H. (Korea University, Seoul, Republic of Korea), Cho, H.Y. (Korea University, Seoul, Republic of Korea), E-mail: hycho@korea.ac.kr
Format:	Artikel
Sprache:	eng
Schlagworte:	catechins cytochrome p450 2E1 ETANOL ETHANOL HepG2 cells
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Zusammenfassung:	To evaluate the toxicological properties of human cytochrome p450 2El (CYP2El) induced by ethanol and possible protective effects of various green tea catechins on alcohol-induced toxicity, transfected HepG2 cells that stably and constitutively express human CYP2El were established using the recombinant retroviral expression vector. Exposure of the CYP2El-expressing HepG2 cells to high concentration of ethanol (200 mM) for 5 days resulted in a more than 50% increase of cytotoxicity, assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction, lactate dehydrogenase (LDH) release, and reactive oxygen species (ROS) production, and loss of normal morphology, in comparison with HepG2 cells containing control vector.
ISSN:	1017-7825