Purification and Characterization of a Cytoscolic Phopholipase A2 from Rat Liver

Purification and Characterization of a Cytoscolic Phopholipase A2 from Rat Liver

A cytosolic phospholipase A2 (PLA2) was purified 640-fold from rat liver by sequential anion-exchange chromatography, Ca2+ -precipitation/KCI-solubilization, gel filtration chromatography, and affinity chromatography.

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Veröffentlicht in:Molecules and cells 2003-12, Vol.16 (3)
Hauptverfasser: Lee, D.K, Won, J.H, Auh, C.K, Park, Y.N. (Sungkyunkwan University, Suwon, Republic of Korea)
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PURIFICATION
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creator Lee, D.K
Won, J.H
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Park, Y.N. (Sungkyunkwan University, Suwon, Republic of Korea)
description A cytosolic phospholipase A2 (PLA2) was purified 640-fold from rat liver by sequential anion-exchange chromatography, Ca2+ -precipitation/KCI-solubilization, gel filtration chromatography, and affinity chromatography.
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source SpringerNature Complete Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry
subjects PURIFICATION
title Purification and Characterization of a Cytoscolic Phopholipase A2 from Rat Liver
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