Purification and Characterization of a Cytoscolic Phopholipase A2 from Rat Liver

A cytosolic phospholipase A2 (PLA2) was purified 640-fold from rat liver by sequential anion-exchange chromatography, Ca2+ -precipitation/KCI-solubilization, gel filtration chromatography, and affinity chromatography.

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Veröffentlicht in:Molecules and cells 2003-12, Vol.16 (3)
Hauptverfasser: Lee, D.K, Won, J.H, Auh, C.K, Park, Y.N. (Sungkyunkwan University, Suwon, Republic of Korea)
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Sprache:eng
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Zusammenfassung:A cytosolic phospholipase A2 (PLA2) was purified 640-fold from rat liver by sequential anion-exchange chromatography, Ca2+ -precipitation/KCI-solubilization, gel filtration chromatography, and affinity chromatography.
ISSN:1016-8478