Effects of load exercises on components of plasma protein and hemoglobin by electrophoretic analysis in rats

Changes in plasma protein and hemoglobin due to exercise were studied by cellulose acetate electophoresis, isoelectric focusing and SDS-polyacrylamide gel electrophoresis. The experiment was carried out on adult male wister rats weighing 206-233g. The method of load exercise used consisted of twenty minutes of swimming. Amounts of plasma protein and hemoglobin increased rapidly immediately after exercise, but there were no significant changes 24 hours after exercise. 1) Plasma protein component: The concentration of albumin and phi+gamma-globulin increased after load exercise on the cellulose acetate electrophoresis. Fractions of pH 6.0 on the isoelectric focusing slightly increased immediately after exercise. 2) Hemoglobin component: Concentrations of fraction II on cellulose acetate electrophoresis and of the fraction of molecular weight 16,000 on SDS-polyacrylamide gel electrophoresis increased immediately after exercise. Significant changes in isoelectric focusing patterns were not observed after exercise