Inhibitory effects of apple polyphenol on induced histamine release from RBL-2H3 cells and rat mast cells

The anti-allergic activities of polyphenol fractions extracted from immature fruits of apple (Rosaceae, Malus sp.) were evaluated by in vitro assays. A crude apple polyphenol (CAP) fraction, which had been obtained from the juice of immature apples by reverse-phase column chromatography, was further...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1998-07, Vol.62 (7), p.1284-1289
Hauptverfasser:	Kanda, T. (Nikka Whisky Distilling Co. Ltd., Kashiwa, Chiba (Japan)), Akiyama, H, Yanagida, A, Tanabe, M, Goda, Y, Toyoda, M, Teshima, R, Saito, Y
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals anti-allergic activity Antigens apple polyphenol APPLES Biflavonoids Biological and medical sciences Calcium - metabolism calcium influx Catechin - pharmacology Chlorogenic Acid - pharmacology Flavonoids Fruit - chemistry General pharmacology HISTAMINA HISTAMINE Histamine Antagonists - isolation & purification Histamine Antagonists - pharmacology histamine release Histamine Release - drug effects hyaluronidase Hyaluronoglucosaminidase - antagonists & inhibitors Hydrolyzable Tannins - isolation & purification Hydrolyzable Tannins - pharmacology HYPERSENSITIVITY Leukemia, Basophilic, Acute MANZANA Mast Cells - drug effects Mast Cells - metabolism Medical sciences p-Methoxy-N-methylphenethylamine Peritoneum - drug effects Peritoneum - metabolism Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Phenols - isolation & purification Phenols - pharmacology Phlorhizin - pharmacology POLIFENOLES Polymers - isolation & purification Polymers - pharmacology POLYPHENOL POLYPHENOLS POMME Proanthocyanidins Rats REACCIONES ALERGICAS REACTION ALLERGIQUE Tumor Cells, Cultured - drug effects
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The anti-allergic activities of polyphenol fractions extracted from immature fruits of apple (Rosaceae, Malus sp.) were evaluated by in vitro assays. A crude apple polyphenol (CAP) fraction, which had been obtained from the juice of immature apples by reverse-phase column chromatography, was further purified by LH-20 column chromatography to obtain an apple condensed tannin (ACT) fraction consisting of linear oligomeric epicatechins from the dimer to pentadecamer. ACT strongly inhibited the release of histamine from rat basophilic leukemia (RBL-2H3) cells stimulated by the antigen-stimulation and from rat peritoneal mast cells stimulated by compound 48/80. The IC50 values for histamine release were 30 mu-g/ml and 25 mu-g/ml, respectively. ACT also inhibited hyaluronidase activity and the increase in intracellular free calcium concentration in RBL-2H3 cells stimulated with the antigen. These results suggest that ACT affected early signal transduction including the calcium influx
ISSN:	0916-8451 1347-6947
DOI:	10.1271/bbb.62.1284