Characterization of insulin like growth factor-1 (IGF-1) partial gene in mithun

A 396 bp fragment of insulin like growth factor-1 (IGF-1) gene, which included complete exon 5, was amplified in mithun (Bos frontalis). The PCRâRFLP analysis showed the absence of polymorphism in this fragment with respect to MspI restriction enzyme and showed 1 restriction site, which produced 2...

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Veröffentlicht in:	Indian journal of animal sciences 2012-01, Vol.82 (1)
Hauptverfasser:	Panigrahi, Manjit, Subodh Kumar, Deb, , S.M, Mitra, Abhijit, Sharma, Arjava, Bujarbaruah, K.M
Format:	Artikel
Sprache:	eng
Schlagworte:	BOVINA BOVINAE FACTEUR DE CROISSANCE IGF FACTOR DE CRECIMIENTO IGF INSULIN-LIKE GROWTH FACTOR POLIMORFISMO POLYMORPHISM POLYMORPHISME
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container_title	Indian journal of animal sciences
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creator	Panigrahi, Manjit Subodh Kumar Deb, , S.M Mitra, Abhijit Sharma, Arjava Bujarbaruah, K.M
description	A 396 bp fragment of insulin like growth factor-1 (IGF-1) gene, which included complete exon 5, was amplified in mithun (Bos frontalis). The PCRâRFLP analysis showed the absence of polymorphism in this fragment with respect to MspI restriction enzyme and showed 1 restriction site, which produced 2 fragments of 273 and 123 bp. Only 2 single nucleotide differences existed in mithun sequences when compared to that of cattle. The genomic sequence homology between mithun and cattle was 99.5%, whereas the deduced amino acid sequences showed 100% homology. The sequence of the wild allele, which was the first report on exon 5 of mithun IGF-1, was submitted to GenBank (Accession number EF686016).
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The PCRâRFLP analysis showed the absence of polymorphism in this fragment with respect to MspI restriction enzyme and showed 1 restriction site, which produced 2 fragments of 273 and 123 bp. Only 2 single nucleotide differences existed in mithun sequences when compared to that of cattle. The genomic sequence homology between mithun and cattle was 99.5%, whereas the deduced amino acid sequences showed 100% homology. 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subjects	BOVINA BOVINAE FACTEUR DE CROISSANCE IGF FACTOR DE CRECIMIENTO IGF INSULIN-LIKE GROWTH FACTOR POLIMORFISMO POLYMORPHISM POLYMORPHISME
title	Characterization of insulin like growth factor-1 (IGF-1) partial gene in mithun
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