Characterization of insulin like growth factor-1 (IGF-1) partial gene in mithun

A 396 bp fragment of insulin like growth factor-1 (IGF-1) gene, which included complete exon 5, was amplified in mithun (Bos frontalis). The PCR–RFLP analysis showed the absence of polymorphism in this fragment with respect to MspI restriction enzyme and showed 1 restriction site, which produced 2 fragments of 273 and 123 bp. Only 2 single nucleotide differences existed in mithun sequences when compared to that of cattle. The genomic sequence homology between mithun and cattle was 99.5%, whereas the deduced amino acid sequences showed 100% homology. The sequence of the wild allele, which was the first report on exon 5 of mithun IGF-1, was submitted to GenBank (Accession number EF686016).