Anthelmintic effect of Zingiber purpureum infuse and extract on adult worms of Haemonchus contortus in vitro

The aim of this study is to determine anthelmintic effect of "bangle" (Zingiber purpureum) on adult worms of Haemonchus contortus in vitro. Bangle were washed and cut into small pieces, and then put into oven with temperature of not more than 50 deg. C for 4 days. Furthermore, it was grained with a mesh of 0.75 mm into a form of powder. Infuse was made by mixing a powder of "bangle" with 100 ml distil water on breaker glass and then heat on water bath for 30 minutes at temperature of 90 deg. C. It was made at a concentration (w/v) of 0 percent, 2.5 percent, 5 percent, 10 percent, 20 percent and 40 percent. Extract was made by percolation with methanol until filtrate produced, and this filtrate was condensed with rotapavor at 48 deg. C with rotated at 200 rpm. Extract were made into a concentration (w/v) of 0 percent, 0.5 percent, 1 percent, 2 percent, 4 percent and 8 percent. Ten adult worms of H. contortus in Petri-dish were dipped with infuse or extract at each concentration with 5 replications. Observations were made every 30 minutes for a period of 6 hours to determine whether the adult worms were still alive or death. Faeces from donor of sheep artificially infected with H. contortus were cultured in vermiculite with infuse or extract used to keep humidity. Each treatment was repeated 5 times. Observations were made at the end of experiment to calculate on the total number infective larvae. The results showed that infuse or extract of "bangle" have anthelmintic effect on H. contortus. The form of extract showed a better effect compared with infuse at a concentration of 0.5 percent and 2.5 percent respectively. The increase of concentration will result in a better effect Tujuan dari penelitian ini adalah untuk mengetahui efek antelmintik dari rimpang bangle (Zingiber purpureum) terhadap cacing Haemonchus contortus secara in vitro. Rimpang bangle setelah dicuci dan dipotong-potong kecil kemudian dimasukkan ke dalam oven dengan suhu tidak lebih dari 50 derajat C selama 4 hari. Rimpang ini kemudian dihaluskan dengan penggiling yang mempunyai ukuran 0,75 mm sehingga menjadi serbuk. Infus dibuat dengan mencampur sejumlah serbuk rimpang bangle dengan 100 ml air suling di dalam panci, panaskan di atas penangas air selama 30 menit dihitung saat suhu mencapai 90 derajat C. Sediaan infus dibuat dengan konsentrasi (berat/volume) 0 persen, 2,5 persen, 5 persen, 10 persen, 20 persen dan 40 persen. Untuk ekstrak rimpang bangle dibuat dengan cara perkolasi sampai did