The study of the pathogenicity of Brucella suis field isolate and transmission ability from pig to human

The aim of this work was to study the pathogenicity of Brucella suis field isolate and its ability to transmit the disease from pig to human. The transmission ability of field isolate of B. suis was studied by using 4 brucellosis negative pigs which were put together with one of the artificially infected pig. The transmission ability of the diseases from pig to human was carried out by collecting blood samples from pigs and from the workers of pig farms and abattoir and tested them with the Rose Bengal Plate Test. The result indicated that field isolate of B. suis biotype 1 was pathogenic for pigs with the ID50 of 500 CFU. The infection was widely distributed into several organs or lymphnodes such as retropharingeal, submaxillaris, femoralis, suprascapularis, supramamaria and the spleen. The transmission of brucellosis from artificially infected pigs to negative pig in the group occurred between 4-6 weeks after they were put together. Brucellosis in the pigs was transmitable and causing brucellosis to workers of pig farms and abattoir Penelitian ini bertujuan untuk mempelajari sifat patogenisitas B. suis isolat lapang dan kemampuan penularannya dari babi kepada manusia. Sifat penularan B. suis pada babi diteliti dengan menggunakan babi negatif sebanyak 4 ekor yang disatukandangkan dengan 1 ekor babi yang telah diinfeksi secara buatan. Sifat penularan brucellosis kepada manusia dipelajari dengan dilakukan pengambilan sampel darah dari kelompok babi baik di peternakan maupun di rumah potong hewan (RPH) dan dari kelompok pekerja kandang dan pekerja rumah potong hewan. Hasil penelitian menunjukkan, bahwa B. suis biotipe 1 isolat lapang merupakan kuman yang patogenik bagi babi, dengan ID50 sebesar 500 CFU. Infeksinya terdistribusi secara luas pada organ atau kelenjar pertahanan tubuh, yaitu limfoglandula (lgl.) retroparingealis, lgl. submaksilaris, lgl. femoralis, lgl. supraskapularis, lgl. supramamaria dan pada limpa. Penularan brucellosis dari babi yang diinfeksi secara buatan melalui selaput lendir mata pada babi sehat dalam kelompoknya terjadi 4-6 minggu setelah inokulasi. Brucellosis pada babi juga terbukti telah menular atau menyebabkan brucellosis pada pekerja kandang dan pekerja rumah potong hewan