In vitro killing assays of antisera antibody sheep post-infected with Fasciola gigantica with the presence of macrophages cells against homologous and heterologous liver flukes

The previous artificial infection known that the Indonesian Thin Tail (ITT) sheep was resistance against the liver fluke of Fasciola gigantica, the resistances occurred in the early infection. In order to observe the immune resistance, some in vitro studies were undertaken in the laboratory, to assay the ability of the antisera antibody of ITT sheep post-infected with F. gigantica, with the presence of macrophages cells in killing the homologous and heterologous liver flukes. The viability of liver flukes were observed within 24-72 hours of incubation period by observing their motility (motile flukes were designated live and non-motile once were death). The results showed that after 72 hours incubation, the motilities of the Newly Excysted Juvenile (NEJ) of F. gigantica incubated with the presence of post-infected sera and macrophages cells solution were significantly lower (P0.05) than that of normal sheep sera. On the contrary, the post-infected sera and macrophages cells solution did not reduce the motilities of the NEJ of F. hepatica, and the death of these flukes were not significantly reduced (P0.05). It seems that the occurrence of homologous antibody to the antigens is very important in the development of killing mechanism. The absence of homologous antibody did not reduce the number of flukes or the ability of macrophages cells in killing F. hepatica was not apparent Berdasarkan penelitian infeksi buatan telah diketahui bahwa domba ekor tipis Indonesia (ITT) resisten terhadap infeksi Fasciola gigantica, dan resistensinya terjadi pada awal infeksi. Untuk mengetahui sifat resistensi tersebut, serangkaian pengamatan secara in vitro dilakukan di laboratorium, untuk menguji daya bunuh antiserum antibodi domba ITT pasca infeksi F. gigantica terhadap cacing hati yang homolog dan heterolog, dengan adanya sel makrofag. Viabilitas cacing diamati dalam waktu inkubasi 24-72 jam, dengan melihat pergerakan cacing (bergerak berarti hidup dan tidak bergerak berarti mati). Hasilnya memperlihatkan bahwa setelah inkubasi selama 72 jam, motilitas Newly Excysted Juvenile (NEJ) F. gigantica dalam larutan yang mengandung serum kebal dan sel makrofag jauh lebih rendah (P0,05) dan mampu menyebabkan kematian NEJ dibandingkan dengan NEJ F. gigantica dalam larutan yang mengandung serum normal dan sel makrofag. Sebaliknya, larutan serum kebal dan sel makrofag tidak mampu menurunkan motilitas NEJ F. hepatica, sehingga penurunan kematian pada NEJ F. hepatica tidak nyata (P0,05)