False positives caused by single primer in DDRT analysis of soybean

[Objective] The aim was to explore the reasons of false positives in Different Display Reverse Transcription (DDRT) analysis. [Method] Soybean varieties “Jilin 30” and “Tongnong 13” were used as materials to carry out analysis on false positives in DDRT analysis. [Result] An important origin of false positives appeared in DDRT analysis was the non-specific amplification caused by the combination of single primer and cDNA. The parallel PCR test of single primer should be set so as to verify whether the obtained fragments were the false positives or the PCR productions combined with single primer. [Conclusion] This study had provided basis for improving the success rate of DDRT experiment.