Optimization of callus induction medium for Schisandga chinensis Baill via uniform design

Optimization of callus induction medium for Schisandga chinensis Baill via uniform design

[Objective] This study was to optimize callus induction medium for Schisandga chinensis Baill. [Method] Using callus induction rate as an indicator, uniform design was employed to optimize hormone combination at poly-factors and poly-levels for callus induction from Schisandga chinensis Baill. [Resu...

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Veröffentlicht in:Hunan agricultural science & technology newsletter : HASTN 2011-01, Vol.12 (1)
Hauptverfasser: Zhang Jia, Agricultural College of Yanbian University, Yanji(China)，Department of Agronomy, Jin Dongchun, Agricultural College of Yanbian University, Yanji(China)，Department of Agronomy, Jin Hailin, Agricultural College of Yanbian University, Yanji(China)，Department of Agronomy
Format: Artikel
Sprache:eng
Schlagworte:
CAL
CALLO
CALLUS
http://www.fao.org/aos/agrovoc#c_24576
http://www.fao.org/aos/agrovoc#c_3842
http://www.fao.org/aos/agrovoc#c_49880
INDUCED MUTATION
MUTACION INDUCIDA
MUTATION PROVOQUEE
TEJIDO
TISSUE
TISSUS
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Zusammenfassung:[Objective] This study was to optimize callus induction medium for Schisandga chinensis Baill. [Method] Using callus induction rate as an indicator, uniform design was employed to optimize hormone combination at poly-factors and poly-levels for callus induction from Schisandga chinensis Baill. [Results] Optimal hormone combinations depended on different explants: optimum medium for both tender leaf and petiole was MS＋3mg/L 6-BA, for stem segment was MS＋3 mg/L 6-BA＋0.6 mg/L NAA. [Conclusion] Uniform design is a time-saving and convenient method for the optimum medium for callus and yields a higher callus induction rate.
ISSN:1009-4229