Study on in vitro bacteriostasis of the different extract from IIex Kudingcha C.J.Tseng

[Objective] The aim was to study bacteriostatic activity of the different extract from IIex Kudingcha C.J.Tseng. [Method] By using test tube 2-fold dilution method and Kirby-Baueer Disc Diffusionto, we conducted inhibitory test on S.aureus and E.coli and determined the minimal inhibitory concentration ( MIC) and minimum bactericidal concentration ( MBC)and the diameter of inhibition zone. [Result] The extract 1 had fairly strong in vitro bacteriostasis activities and than the extract2 and extract3. The MIC and MBC was 3.91mg/ml and 31.25mg/ml on S.aureus and E.coli. [Conclusion] The result showed that the different extract from IIex Kudingcha C.J.Tseng had varying amount of different bacteriostatic activities,the IIex Kudingcha C.J.Tseng was developed new and safe bacteriostatic reagent to provide reference.