Histomorphometry of the uterine horn of queens (Felis catus) uterus submitted to ovaryhisterectomy

Histomorphometric data were obtained from cats uterine horns: either nulliparous (n = 6), primiparous (n=6), multiparous (n = 6) and treated with contraceptive (n = 6). The material was collected after surgery, fixed in paraformaldehyde and embedded in paraplast® resin to be sliced in a microtome. T...

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Veröffentlicht in:Brazilian journal of veterinary research and animal science 2012-09, Vol.49 (3), p.225-231
Hauptverfasser: Cristina Maria Rodrigues Monteiro, Silvia Helena Venturoli Perri, Roberto Gameiro de Carvalho, Adelina Maria da Silva, Marion Burkhardt Koivisto
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Sprache:por
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Zusammenfassung:Histomorphometric data were obtained from cats uterine horns: either nulliparous (n = 6), primiparous (n=6), multiparous (n = 6) and treated with contraceptive (n = 6). The material was collected after surgery, fixed in paraformaldehyde and embedded in paraplast® resin to be sliced in a microtome. The obtained sections were stained with hematoxylin - eosin and measured under a light microscope: uterine wall total thickness (μm), endometrium total height, endometrial glands diameter and glandular epithelia height, total myometrium, internal and external myometrium and vascular layer thickness. It was concluded that: 1 - contraceptive use and number of pregnancies altered the uterine structure, 2 - one pregnancy does not appear to affect the uterine lining structures as occurs in multiparous cats, 3 – there was no variation on the evaluated structures between nulliparous and primiparous cats except for inner myometrium, 4 - the total myometrium hight and the endometrium hight showed similar variations except for the contraceptive treated cats group, 5 - the outer myometrium showed marked changes in the treated cats, 6 – the less marked morphological variations were for the endometrial glands diameter and glandular epithelium hight, 7 - the presence of dilated endometrial glands was found only in treated cats. Dados histomorfométricos foram obtidos de cornos uterinos de gatas nulíparas (n=6), primíparas (n= 6), multíparas (n=6) e de gatas tratadas com contraceptivo (n=6).O material coletado após a cirurgia foi fixado em paraformaldeído e incluído em resina Paraplast® para se proceder a microtomia. Os cortes adquiridos foram submetidos à coloração rotineira com hematoxilina e eosina e examinados ao microscópio de luz para a realização das seguintes mensurações:espessura total (μm) da parede uterina, endométrio total, diâmetro das glândulas endometriais e altura dos respectivos epitélios glandulares, espessura total do miométrio, do miométrio interno,miométrio externo e estrato vascular.Concluiu-se que: uso de anticoncepcionais e inúmeras gestações alteraram a estrutura uterina; uma única gestação parece não afetar significativamente as estruturas da parede uterina como ocorre nas gatas multíparas; não houve variação significativa das estruturas entre as gatas nulíparas e primíparas, com exceção para miométrio interno; o miométrio total e o endométrio apresentaram variações semelhantes, com exceção para o grupo das gatas tratadas; o miométrio externo apresentou
ISSN:1413-9596
1678-4456