Localization and activity of tissue lipoprotein lipase in feline prostate gland

Tissue lipoprotein lipase - LPL activity has been studied in prostate glands of 7 adult male European shorthair cats on cryostat cross sections by the Tween technique. The highest activity was observed at the luminal surface of the glandular epithelium, its apical parts, as well as in the lumen of glandular tubules of prostate body. A moderate intensity of LPL expression was found out in the basal parts of parenchymal epithelial cells. The enzyme was slightly expressed in the stroma of the gland, whereas no LPL activity was detected in the glandular capsule. The results provided evidence for a predilection in tissue LPL localization in epithelial components of the glands, as well as for higher enzyme activity in the luminal part of the epithelium, thus presuming a role of LPL in the lipid metabolism of glandular parenchyma, in the production and excretion of lipid products into the tubuloalveolar lumen.