Method and media for enhancing viability, maturation, and cryopreservation of cells

A method of enhancing the viability of cryopreserved cells is culturing Sertoli cells in media to produce preconditioned media and adding the preconditioned media to the cells to be cryopreserved. The cells are then cryopreserved. Alternatively, a method of enhancing the viability of cryopreserved c...

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Bibliographische Detailangaben
Hauptverfasser: DON F. CAMERON, SAMUEL SAPORTA, AGNETA OTHBERG, PAUL R. SANBERG, CESARIO V. BORLONGAN
Format: Patent
Sprache:eng
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Zusammenfassung:A method of enhancing the viability of cryopreserved cells is culturing Sertoli cells in media to produce preconditioned media and adding the preconditioned media to the cells to be cryopreserved. The cells are then cryopreserved. Alternatively, a method of enhancing the viability of cryopreserved cells is co-culturing Sertoli cells and cells to be cryopreserved in media and cryopreserving both.