TISSUE CULTURE AND RAPID PROPAGATION METHOD OF RHODODENDRON DENUDATUM ASEPTIC SEEDLINGS

The invention provides a tissue culture and rapid propagation method of Rhododendron denudatum aseptic seedlings, and relates to the technical field of plant tissue culture and rapid propagation. The method of the invention comprises the following steps: taking young tissues of Rhododendron denudatum as explants, sterilizing the explants, inoculating the explants on an induction medium, and inducing and culturing for more than 30 days to obtain sterile seedlings of Rhododendron denudatum; the induction medium takes 1/2WPM as the basic medium, and further comprises 1.5mg/L 6-BA, 1.0mg/L 2,4-D, 50mg/L hydrolyzed casein, 30g/L sucrose and 8g/L agar powder, and the pH value is 5.0. The method provided by the invention is simple to operate, and can make the emergence rate as high as 87 percent, thus solving the existing problem of low reproductive efficiency of Rhododendron denudatum.