OPTIMIZED METHOD FOR EXTRACTING RNA OF MILK FAT

Provided is an optimized method for extracting RNA of milk fat, falling within the field of molecular genetic breeding of dairy cows. The optimized method for extracting RNA of milk fat includes the following steps: S1. sample acquisition; S2. homogenization; S3. upper lipid layer removal; S4. incub...

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Hauptverfasser:	Zhang Taoping, Yang Benshun, Qiu Liang, Yang Wucai, Tan Jianbing, Zan Linsen
Format:	Patent
Sprache:	eng
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Beschreibung
Zusammenfassung:	Provided is an optimized method for extracting RNA of milk fat, falling within the field of molecular genetic breeding of dairy cows. The optimized method for extracting RNA of milk fat includes the following steps: S1. sample acquisition; S2. homogenization; S3. upper lipid layer removal; S4. incubation; S5. isolation; S6. uniform mixing; S7. re-centrifugation; S8. precipitate washing; S9. centrifugation for purification; S10. air-drying and dissolving of RNA; and S11. final assay. After the pyrolysis by a Trizol reagent, the milk fat is re-centrifuged to ensure the purify of the RNA extracted. In the process of precipitation of RNA by an isopropyl alcohol solution, RNase-free Glycogen reagent is added to improve the concentration, purify, and integrity of RNA.