A COMPOSITION FOR ANTI-OVARIAN AGING OF COWS AND APPLICATION THEREOF

The invention discloses a composition and an additive made from the composition for anti-ovarian aging of cows and its application. The composition is made of anthocyanin, glycyrrhetinic acid and lycium barbarum glycopeptide. The invention discloses that the composition, in clinical treatment of cow ovarian aging, could result in an increase in splenic germinal centres; and reveals the mechanism of the antioxidant effect of the composition on primary ovarian granulosa cells in cows, mainly be participating in the regulation of programmed cell death, Ras protein signaling, tissue development, cellular serine and tryptophan kinase activities, MAPK signaling pathway, PI3K-AKT signaling pathway. Laser confocal results showed that the composition could significantly reduce the generation of mitochondrial reactive oxygen species; at the same time, the therapeutic effect on the persistent corpus luteum of the cows was achieved by improving the structure of the intestinal flora, and the colony was significantly changed at the upstream of the genus level; B-ultrasound results revealed that more large follicles could be detected in the ovaries of persistent luteal cows after one month of administration of the composition.