METHOD FOR PURIFYING BIOLOGICALLY ACTIVE PEPTIDE BY USING PROTEIN A AFFINITY CHROMATOGRAPHY

The present invention provides a method for purifying an Fc-containing biologically active peptide mixture by using an affinity column comprising an affinity matrix that contains a protein A ligand, the mixture comprising a first Fc-containing biologically active peptide and a second Fc-containing biologically active peptide, wherein the second Fc-containing biologically active peptide comprises at least one more human VH3 domain than the first Fc-containing biologically active peptide. According to the purification method, biologically active peptides having the same or similar structures can be precisely separated with high purity, while process simplification is achieved.