DNA SASTAV PROTEIN KOMPLEKSA I POSTUPAK ZA DOBIJANJE REKOMBINOVANOG PROTEIN KOMPLEKSA

Pronalazak se odnosi na DNA sastav protein kompleksa i postupak za dobijanje rekombinovanog protein kompleksa, gde DNA sastav protein kompleksa enkodira aktivnost protein kompleksa koji sadrži ljudski faktor VIII: C, s tim što taj protein kompleks obuhvata prvi polipeptid homologan ljudskom faktoru...

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Hauptverfasser:	CHAPMAN, B, BURKE, L.R, MIKKELSEN, M.J, RASMUNSSEN, E.M
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Pronalazak se odnosi na DNA sastav protein kompleksa i postupak za dobijanje rekombinovanog protein kompleksa, gde DNA sastav protein kompleksa enkodira aktivnost protein kompleksa koji sadrži ljudski faktor VIII: C, s tim što taj protein kompleks obuhvata prvi polipeptid homologan ljudskom faktoru VIII: C domena A, koji je povezan sa drugim polipeptidom homolognim domenu C ljudskog faktora VIII: C preko polipeptidskog prostora a ne celom dužinom domena B ljudskog faktora pri čemu DNA sastav obuhvata kontrolne sekvence pogodne za usmeravanje ekspresije protein kompleksa kada je DNA sastav prisutan u domaćoj eukariotik ćeliji. Postupak za dobijanje rekombinovanog protein kompleksa koji obuhvata odgajivanje doraaćih ćelija pod uslovima u kpjima je izražen DNA sastav koji je enkodirao protein kompleks. Recombinant protein complexes having human Factor VIII:C activity are produced in a eukaryotic host cell by expressing a DNA construct encoding said protein complex joined in reading frame with control sequences providing for the expression of said DNA construct in said host cell, wherein said DNA construct comprises a nucleotide sequence that encodes a first polypeptide homologous to the A domain of human Factor VIII:C linked to a second polypeptide homologous to the C domain of human Factor VIII:C by a polypeptide spacer, wherein the polypeptide spacer is other than the full length B domain of human Factor VIII:C and comprises an amino acid sequence homologous to a human Ig heavy chain hinge region.