PROCESS FOR TRANSFERRING GENES INTO PLANTS

Postupak za genski transfer kod biljaka, naznačen time, što se a) iz prašnika prebace nezrela polenska zrna u granljivi rastvor i ukloni okružujuće tkivo, b) izdvojena polenska zrna gaje u granljivom rastvoru, c) tuđi genski materijal prenosi in vitro u polenska zrna tokom gajenja i sazrevanja, d) t...

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Bibliographische Detailangaben
Hauptverfasser: HEBERLE-BORS, ERWIN DR, BENITO, MORENO ROSA MARIA, ALWEN, ANNA
Format: Patent
Sprache:eng
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Zusammenfassung:Postupak za genski transfer kod biljaka, naznačen time, što se a) iz prašnika prebace nezrela polenska zrna u granljivi rastvor i ukloni okružujuće tkivo, b) izdvojena polenska zrna gaje u granljivom rastvoru, c) tuđi genski materijal prenosi in vitro u polenska zrna tokom gajenja i sazrevanja, d) transformisana polenska zrna drže in vitro do potpunog sazrevanja, e) biljke-primaoci oprašuju transformisanim genskim polenskim zrnima i iz njih se dobije seme. - Prijava sadrži još 7 zavisnih zahteva. In a process for gene transfer in plants, a) unripe pollen grains are removed from the stamens, placed in nutrient solution, and the surrounding tissue is removed, b) the isolated unripe pollen grains are cultured in nutrient solution, c) exogenous genetic material is transferred to the pollen grains during the in vitro culturing and ripening, d) the transformed pollen grains are fully ripened in vitro, e) the receiver plants are pollenated with the transformed pollen grains and their seeds are collected. The invention also concerns transgenic seeds and reproduction products.