ENZYMATICALLY-ACTIVE RECOMBINANT HUMAN beta -TRYPTASE AND METHOD OF MAKING SAME

Disclosed is a method of expressing enzymatically-active, recombinant human beta -tryptase in a yeast host cell, expression constructs which drive the production of enzymatically-active tryptase in transformed yeast, and genetically-engineered yeast containing the expression constructs and which exp...

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Bibliographische Detailangaben
Hauptverfasser:	MAFFITT, MARK, A, NILES, ANDREW, L, HAAK-FRENDSCHO, MARY
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed is a method of expressing enzymatically-active, recombinant human beta -tryptase in a yeast host cell, expression constructs which drive the production of enzymatically-active tryptase in transformed yeast, and genetically-engineered yeast containing the expression constructs and which express enzymatically-active human beta -tryptase. Uses for the tryptase so produced are also disclosed. L'invention a trait à une méthode d'expression de la beta -tryptase humaine de recombinaison active du point de vue enzymatique dans une cellule de levure hôte, à des produits de recombinaison d'expression commandant la production de cette tryptase dans la levure transformée ainsi qu'à de la levure génétiquement modifiée contenant ces produits de recombinaison d'expression et exprimant la beta -tryptase humaine de recombinaison active du point de vue enzymatique. Cette invention concerne également des applications de la tryptase ainsi produite.