CEPHALOSPORIN DEACETYLASE, GENE CODING FOR IT, AND PREPARATION METHOD OF DEACETYLATED CEPHALOSPORIN COMPOUNDS USING IT

An isolated cephalosporin deacetylase derived from a strain of Bacillu sp. which (i) has an optimum pH between 8.0 and 9.0; (ii) has an optimum temperature of about 40 DEG C; and (iii) has an isoelectric point of about 4.3, and a DNA sequence which codes for the cephalosporin deacetylase are disclos...

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Bibliographische Detailangaben
Hauptverfasser:	LIM, BUN, SAM, KIM, YONG, DUK, LEE, SANG, HOON, JEONG, IL, SEON, CHOI, DUK, HO, HAN, KUM, SOO
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Zusammenfassung:	An isolated cephalosporin deacetylase derived from a strain of Bacillu sp. which (i) has an optimum pH between 8.0 and 9.0; (ii) has an optimum temperature of about 40 DEG C; and (iii) has an isoelectric point of about 4.3, and a DNA sequence which codes for the cephalosporin deacetylase are disclosed. An expression vector comprising the DNA sequence and transformed host strain carrying the vector are provided and used to deacetylate cephalosporin compounds to give useful intermediates for cephalosporin antibiotics synthesis. L'invention concerne une céphalosporine désacétylase dérivée d'une souche de Bacillu sp. qui présente (i) un pH optimal se situant entre 8,0 et 9,0.(ii) une température optimale d'environ 40 DEG C et (iii) un point isoélectrique d'environ 4,3. L'invention concerne également une séquence d'ADN codant pour cette céphalosporine désacétylase ainsi qu'un vecteur d'expression comprenant cette séquence d'ADN et une souche hôte transformée portant ce vecteur, ces derniers étant utilisés pour désacétyler les composés de céphalosporine ce qui permet d'obtenir des intermédiaires utiles pour la synthèse d'antibiotiques céphalosporine.