ASSAYS AND PROBES WITH ENZYME LABELS

Probes comprise S1 and P1 nuclease (as an enzyme label) linked to a specific binding member such as a nucleotide sequence or an antibody. Such probes are useful for sandwich assays. As compared with known probes using alkaline phosphatase as a label, advantages include relative insensitivity to phos...

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Bibliographische Detailangaben
Hauptverfasser:	FISHER, MARK, HARBRON, STUART, TAYLORSON, CHRISTOPHER, JOHN
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Probes comprise S1 and P1 nuclease (as an enzyme label) linked to a specific binding member such as a nucleotide sequence or an antibody. Such probes are useful for sandwich assays. As compared with known probes using alkaline phosphatase as a label, advantages include relative insensitivity to phosphate and elevated temperature and reduced risk of nonspecific binding. Des sondes comprenant les nucléases S1 et P1 (comme enzymes marqueurs), liées à un élément de liaison spécifique tel qu'une séquence nucléotidique ou un anticorps, sont utiles pour les méthodes de dosage en sandwich. Par comparaison avec les sondes connues utilisant comme marqueurs les phosphatases alcalines, ces sondes ont pour avantages une insensibilité relative aux phosphates et à l'élévation de température, ainsi qu'un risque moindre de liaison non spécifique.