CONJUGATION OF LIGAND TO IMMOBILIZED PROTEIN IN ORGANIC SOLVENT

A new method for immobilization of small molecules on solid supports via a macromolecular spacer has been developed. A protein or another macromolecule is first immobilized in an aqueous medium, and the solid support is then washed with an organic solvent. The small molecule is coupled in an organic...

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Bibliographische Detailangaben
Hauptverfasser:	SUNSHINE, EDWARD, PIRAN, URI, PETERSON, JAMES, W, CHANG, STEVE, C., S, RIORDAN, WILLIAM, J
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY CHEMISTRY GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY ORGANIC CHEMISTRY PEPTIDES PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS TECHNICAL SUBJECTS COVERED BY FORMER USPC TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ARTCOLLECTIONS [XRACs] AND DIGESTS TESTING THEIR RELEVANT APPARATUS TRANSPORTING
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Beschreibung
Zusammenfassung:	A new method for immobilization of small molecules on solid supports via a macromolecular spacer has been developed. A protein or another macromolecule is first immobilized in an aqueous medium, and the solid support is then washed with an organic solvent. The small molecule is coupled in an organic medium, followed by organic medium washes. The new solid phases are useful for affinity purifications, immunoassays and other binding assays, and for selection of binders by panning procedures. Nouvelle méthode d'immobilisation de petites molécules sur des supports solides via un bras d'espacement macromoléculaire. Une protéine ou une autre macromolécule est d'abord immobilisée en milieu aqueux, puis le support solide est lavé à l'aide d'un solvant organique. La petite molécule est couplée dans un milieu organique, puis des lavages en milieu organique sont opérés. Les nouvelles phases solides sont utiles pour les purifications par affinité, les immuno-essais et autres analyses par liaison, ainsi que pour la sélection de liants par des méthodes d'adhérence sur plastique.