METHODS FOR ISOLATION OF MOST ABUNDANT OLIGONUCLEOTIDES FROM COMPLEX MIXTURES

Double-stranded nucleic acids present in a mixture thereof are converted to the individual strands thereof which are renatured under conditions which favors reannealing of the nucleic acids present at higher than average concentrations in the original mixture. The procedure can be used for identifyi...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	BERTELSEN, ARTHUR, H, FATHI, REZA, BEUTEL, BRUCE, A, SHERMAN, MICHAEL, I, RUDOLPH, M., JONATHAN, COOK, ALAN, F, COPPOLA, GEORGE, R, GAO, HETIAN
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES DERIVATIVES THEREOF ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS SUGARS THEIR RELEVANT APPARATUS TRANSPORTING VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Double-stranded nucleic acids present in a mixture thereof are converted to the individual strands thereof which are renatured under conditions which favors reannealing of the nucleic acids present at higher than average concentrations in the original mixture. The procedure can be used for identifying nucleic acids which bind to a target molecule or other compounds which bind to a target molecule by using nucleic acids as a coding portion of a chimeric molecule which includes such compounds. Des acides nucléiques double-brin présents dans un mélange desdits acides sont convertis en leurs brins individuels qui sont renaturés dans des conditions favorisant la recirculation des acides nucléiques présents à des concentrations supérieures à la moyenne dans le mélange d'origine. On peut utiliser le procédé pour identifier des acides nucléiques se fixant à une molécule cible ou d'autres composés se fixant à une molécule cible en utilisant des acides nucléiques en tant que partie codante d'une molécule chimère comprenant de tels composés.