DNA ENCODING MURINE PRECURSOR INTERLEUKIN 1 beta CONVERTING ENZYME

Complementary DNA (cDNA) encoding the murine precursor interleukin-1 beta (pre-IL-1 beta ) converting enzyme (ICE) is isolated from a murine cDNA library. A cDNA, encoding the full-length open reading frame (ORF) for the nascent ICE as well as the 20 kDa subunit and the 10 kDa subunit of ICE are identified and sequenced. ICE is useful in the conversion of pre-IL-1 beta into mature IL-1 beta . The murine ICE cDNA is useful in obtaining the genomic sequence. The recombinantly produced murine ICE is useful in the identification of inhibitors of ICE activity. Complementary DNA (cDNA) encoding the murine precursor interleukin-1(beta) (pre-IL-1(beta)) converting enzyme (ICE) is isolated from a murine cDNA library. A cDNA, encoding the full-length open reading frame (ORF) for the nascent ICE as well as the 20 kDa subunit and the 10 kDa subunit of ICE are identified and sequenced. ICE is useful in the conversion of pre-IL-1(beta) into mature IL-1(beta). The murine ICE cDNA is useful in obtaining the genomic sequence. The recombinantly produced murine ICE is useful in the identification of inhibitors of ICE activity. On isole d'une banque d'ADNc murin l'ADN complémentaire (ADNc) codant l'enzyme de conversion (ICE) de l'interleukine-1beta précurseur (pre-IL-1beta) murine. On identifie et on séquence un ADNc codant la totalité de la longueur du cadre de lecture ouvert (ORF) de l'ICE naissant, ainsi que la sous-unité de 20 kDa et la sous-unité de 10 kDa de ICE. L'ICE est efficace dans la conversion de pre-IL-1beta en IL-1beta mature. L'ADNc d'ICE murin est efficace pour obtenir la séquence génomique. L'ICE murin produit par recombinaison est utile dans l'identification d'inhibiteurs de l'activité de l'ICE.