METHOD FOR IMMOBILIZING DYE ON SUBSTRATES

The present invention provides a matrix which can be used to detect and measure quantitatively, accurately and precisely high levels of hydrogen peroxide. The matrix has a large amount of indicators chemically bound thereto. These high levels of hydrogen peroxide may result from the enzyme-mediated...

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Bibliographische Detailangaben
Hauptverfasser:	GIBBONI, DAVID, J, LAW, WAI, TAK
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS BASED THEREON COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES DERIVATIVES THEREOF ENZYMOLOGY GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC MACROMOLECULAR COMPOUNDS PHYSICS POLYSACCHARIDES PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION TECHNICAL SUBJECTS COVERED BY FORMER USPC TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ARTCOLLECTIONS [XRACs] AND DIGESTS TESTING THEIR PREPARATION OR CHEMICAL WORKING-UP VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides a matrix which can be used to detect and measure quantitatively, accurately and precisely high levels of hydrogen peroxide. The matrix has a large amount of indicators chemically bound thereto. These high levels of hydrogen peroxide may result from the enzyme-mediated decomposition of various analytes from undiluted whole blood samples. Both a dyed film-forming polymer and a dyed film-opening polymer are used in combination to provide a reagent film that changes color indicating the presence of hydrogen peroxide. A preferred two component dye system is primaquine immobilized to the film-opening solid matrix particles and MBTH (3-methyl-2-benzothiazolinone-hydrazone) immobilized to the film former. La présente invention se rapporte à une matrice qui peut être utilisée pour détecter et mesurer de manière quantitative et précise des niveaux élevés de peroxyde d'hydrogène. La matière possède une grande quantité d'indicateurs liés chimiquement à celle-ci. Ces niveaux élevés de peroxyde d'hydrogène peuvent résulter d'une décomposition à médiation enzymatique de divers analytes à partir d'échantillons de sang entier non dilué. Un polymère teint formant un film et un polymère teint à ouverture de film sont utilisés en combinaison pour produire un film réactif qui modifie la couleur indiquant la présence du peroxyde d'hydrogène. Un système de teinture préféré à deux composants est fixé par la primaquine aux particules matricielles solides d'ouverture de film et par MBTH(3-méthyl-2-benzothiazolénone-hydrazone) à l'agent filmogène.