PRODUCTION FO TRANSGENIC SOYBEAN PLANTS

A method of generating transgenic soybean plants comprising the steps of: preparing protoplasts from soybean cotyledons; inserting foreign DNA into the prepared protoplasts by electroporation, the foreign DNA comprising a useful gene; culturing the electroplated protoplasts in medium to induce cell...

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Bibliographische Detailangaben
Hauptverfasser:	DHIR, SARWAN, K, WIDHOLM, JACK, M
Format:	Patent
Sprache:	eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method of generating transgenic soybean plants comprising the steps of: preparing protoplasts from soybean cotyledons; inserting foreign DNA into the prepared protoplasts by electroporation, the foreign DNA comprising a useful gene; culturing the electroplated protoplasts in medium to induce cell growth, colony formation and calli generation; and regenerating plants from calli is disclosed. Un procédé de production d'espèces de soja transgéniques comprend les étapes suivantes: préparation de protoplastes à partir de cotylédons de soja; insertion par électroporation d'un ADN étranger dans les protoplastes préparés, ledit ADN comprenant un gène utile; culture des protoplastes électroplaqués dans un milieu pour induire la croissance des cellules, la formation de colonies et la génération de cal; et regénération de plantes à partir dudit cal.