SUBSTRATE FOR POLYMERASE ACTIVITY DETERMINATION

The invention relates to the use of an iodine-2'-deoxyuridine triphosphate or substrate analogue thereof as a substrate in quantitative determination of nucleic acid polymerase activity. The invention also relates to a method of quantitatively determining nucleic acid polymerase activity, compr...

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Bibliographische Detailangaben
Hauptverfasser:	NEUMUELLER, THORD, MAGNUS, GRONOWITZ, JAN-SIMON, LAANGSTROEM-PERSSON, ULLA, M, KAELLANDER, CLAS, FREDRIK, RUNESSON, KARLSTROEM, ROLF, ANDERS
Format:	Patent
Sprache:	eng
Schlagworte:	ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS,e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES,DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS CONVEYING DERIVATIVES THEREOF ENZYMOLOGY HANDLING THIN OR FILAMENTARY MATERIAL INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MAKING ARTICLES OF PAPER, CARDBOARD OR MATERIAL WORKED IN A MANNERANALOGOUS TO PAPER MAKING CONTAINERS OF PAPER, CARDBOARD OR MATERIAL WORKED IN AMANNER ANALOGOUS TO PAPER MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PACKAGES PACKAGING ELEMENTS PACKING PERFORMING OPERATIONS PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDEDFOR SHAPING OR JOINING OF PLASTICS SPIRITS STORING SUGARS TESTING TRANSPORTING VINEGAR WINE WORKING OF PLASTICS WORKING OF SUBSTANCES IN A PLASTIC STATE, IN GENERAL WORKING PAPER, CARDBOARD OR MATERIAL WORKED IN A MANNER ANALOGOUSTO PAPER
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Beschreibung
Zusammenfassung:	The invention relates to the use of an iodine-2'-deoxyuridine triphosphate or substrate analogue thereof as a substrate in quantitative determination of nucleic acid polymerase activity. The invention also relates to a method of quantitatively determining nucleic acid polymerase activity, comprising measurement of the incorporation of a I-labeled iodine-2'-deoxyuridine triphosphate or substrate analogue thereof into a template. The invention further relates to a kit for determining polymerase activity or its blocking antibody comprising a I-labeled iodine-2'-deoxyuridine triphosphate or substrate analogue thereof, a free or immobilized template, and optionally a primer. L'invention a pour objet l'utilisation d'un tri-phosphate d'iode-2'-désoxyuridine ou d'un substrat analogue de celui-ci en tant que substrat utilisé dans la détection quantitative de l'activité de polymérase d'acide nucléique. L'invention concerne également un procédé de détection quantitative d'activité de polymèrase d'acide nucléique qui consiste à mesurer l'introduction d'iode-2'-désoxyuridine triphosphate marqué 125I ou un substrat analogue de celui-ci dans un gabarit. L'invention se rapporte de plus à un dispositif de détection de l'activité de polymérase ou de son anticorps de blocage comprenant de l'iode-2'-désoxyuridine triphosphate marqué 125I ou un substrat analogue de celui-ci, un gabarit libre ou immobilisé, et optionnellement une amorce.