PROCESS FOR RAPID NUCLEIC ACID DETECTION

A nucleic acid probe assay which combines nucleic acid amplification with solution hybridization using capture and reporter probes followed by immobilization on a solid support is provided. An assay sensitivity equal to 5 copies of HIV I DNA was achieved. On a mis au point une analyse par sonde d�...

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Bibliographische Detailangaben
Hauptverfasser:	MILLER, JEFFREY, ALLAN, ADLER, KARL, E, KASILA, PATRICIA, ANN, GREENE, RICHARD, ALFRED
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A nucleic acid probe assay which combines nucleic acid amplification with solution hybridization using capture and reporter probes followed by immobilization on a solid support is provided. An assay sensitivity equal to 5 copies of HIV I DNA was achieved. On a mis au point une analyse par sonde d'acide nucléique, combinant l'amplification d'acide nucléique avec l'hybridation de solutions au moyen de sondes de capture et de sondes rapporteuses, après quoi on procède à l'immobilisation sur un support solide. On a atteint une sensibilité d'analyse égale à 5 copies d'ADN de VIH I.