ENHANCED BIOACTIVITY OF MAMMALIAN SOMATOTROPIN THROUGH SELECTIVE DEAMIDATION

Deamidated forms of mammalian somatotropins originally having an asparagine located between residues 96-101. These deamidated somatotropins have an increased bioactivity as compared to their native species. This invention particularly discloses a composition comprised of bovine somatotropin (bSt) co...

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Bibliographische Detailangaben
Hauptverfasser:	HOOGERHEIDE, JOHN, G, HARBOUR, GARY, C, GARLICK, ROBERT, L
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Deamidated forms of mammalian somatotropins originally having an asparagine located between residues 96-101. These deamidated somatotropins have an increased bioactivity as compared to their native species. This invention particularly discloses a composition comprised of bovine somatotropin (bSt) compounds wherein the bSt has been modified by deamidation from the native state comprising a predominant electrophoretic species having an isoelectric point (pI) of 8.2 to a bioenhanced composition of bSt molecules having a predominant electrophoretic species with pI between 7.5 and 6.5. The preferred bovine somatotropin is that species in which the asparagine located between amino acid residues 96-101 has been modified to isoaspartic acid. Processes for the production of these bioenhanced compositions and for their use are also disclosed.