METHOD OF PRODUCING HIGH MOLECULAR WEIGHT SODIUM HYALURONATE BY FERMENTATION OF STREPTOCOCCUS

A novel mutant microorganism $i(Streptococcus zooepidemicus) HA-116 ATCC 39920. The microorganism produces large amounts of high molecular weight hyaluronic acid. The invention provides a method of obtaining such microorganisms. The invention also concerns a method of obtaining sodium hyaluronate wh...

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Hauptverfasser:	NIMROD, ABRAHAM, GREENMAN, BENJAMIN, KANNER, DOV, LANDSBERG, MOSHE
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS BASED THEREON COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC MACROMOLECULAR COMPOUNDS POLYSACCHARIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS SPIRITS THEIR PREPARATION OR CHEMICAL WORKING-UP VINEGAR WINE
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Beschreibung
Zusammenfassung:	A novel mutant microorganism $i(Streptococcus zooepidemicus) HA-116 ATCC 39920. The microorganism produces large amounts of high molecular weight hyaluronic acid. The invention provides a method of obtaining such microorganisms. The invention also concerns a method of obtaining sodium hyaluronate which comprises growing with vigorous agitation a microorganism of the genus $i(Streptococcus) under appropriate conditions in a suitable nutrient medium containing a sugar component as a carbon source. The sugar component is present in a substantially constant concentration between 0.2 and 10 grams per liter. The medium has a substantially constant pH between about 6.0 and 7.5 and includes a substantially constant magnesium ion concentration above 0.05 grams per liter. The sodium hyaluronate excreted into the medium by the organism is purified using methods involving precipation, redissolving and reprecipating the hyaluronate. Composition of sodium hyaluronate which are characterized by an absence of pyrogenicity and skin irritation are obtained. Un nouveau microorganisme surtout, le Streptococcus zooepidemicus HA-116 ATCC 39920, produit de grandes quantités d'acide hyalyronique de poids moléculaire élevé. L'invention décrit un procédé permettant d'obtenir ces microorganismes, ainsi qu'un procédé de production d'hyaluronate de sodium consistant à cultiver sous agitation vigoureuse un microorganisme du genre Streptococcus dans des conditions et dans un milieu nutritif appropriés contenant un composant de sucre comme source de carbone. Le composant du sucre a une teneur sensiblement constante comprise entre 0,2 et 10 grammes par litre. Le milieu présente un pH sensiblement constant compris entre 6,0 et 7,5 et comprend une teneur en ions magnésium sensiblement constante et supérieure à 0,05 grammes par litre. L'hyaluronate de sodium excrété dans le milieu par l'organisme est purifié par des procédés consistant à précipiter, redissoudre et reprécipiter l'hyaluronate. On obtient ainsi des compositions à base d'hyaluronate de sodium caractérisées par l'absence de pyrogénicité et n'irritant pas la peau.