A PROCESS FOR THE PREPARATION OF HUMAN INSULIN

Human insulin is prepared by treatment of a porcine insulin product, preferably raw porcine insulin, with carboxypeptidase A or a corresponding enzyme to form a desalanine-B30 insulin product, which is condensed, without the addition of a buffer, with a L-threonine alkyl ester in a mixture of a lowe...

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Bibliographische Detailangaben
Hauptverfasser:	BALSCHMIDT, PER, ANDRESEN, FINN, HEDE, HEJNAES, KIM, RY
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION ORPROCESSING OF GOODS ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES SPIRITS TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE VINEGAR WINE
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Beschreibung
Zusammenfassung:	Human insulin is prepared by treatment of a porcine insulin product, preferably raw porcine insulin, with carboxypeptidase A or a corresponding enzyme to form a desalanine-B30 insulin product, which is condensed, without the addition of a buffer, with a L-threonine alkyl ester in a mixture of a lower alkanol and water trypsin, a trypsin derivative or an enzyme related thereto at a pH value of from about 5.5 to about 7.5 and at a temperature between 0 and -30 C to form an insulin alkyl ester, which is hydrolyzed in an aqueous medium at a pH value of from about 8.5 to about 10.5. On prépare de l'insuline humaine en traitant un produit de l'insuline porcine, de préférence de l'insuline porcine brute, avec la carboxypeptidase (A) ou une enzyme correspondante afin de former un produit d'insuline désalanine-B (30), qui est condensé, sans l'addition d'un tampon avec un alkylester de L-thréonine dans un mélange d'alkanol inférieur et d'eau contenant de la trypsine, un dérivé de trypsine ou une enzyme en rapport avec elle à un pH allant d'environ 5,5 à environ 7,5 et à une température située entre 0O et -30OC, afin de former un alkylester d'insuline qui est hydrolisé dans un milieu aqueux à un pH allant d'environ 8,5 à environ 10,5.