PREPARATION METHOD FOR SINGLE-STRANDED CIRCULAR DNA WITH ACCURATE SITE-DIRECTED MODIFICATION

Provided is a preparation method for a single-stranded circular DNA. According to the method, by means of the design of a third DNA fragment and a first DNA fragment, chemical modification of any accurate site in a single-stranded circular DNA can be realized, so that the single-stranded circular DN...

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Bibliographische Detailangaben
Hauptverfasser:	TIAN, Baolei, DONG, Yuanchen
Format:	Patent
Sprache:	chi ; eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Provided is a preparation method for a single-stranded circular DNA. According to the method, by means of the design of a third DNA fragment and a first DNA fragment, chemical modification of any accurate site in a single-stranded circular DNA can be realized, so that the single-stranded circular DNA has higher stability, better binding capacity, improved immunogenicity and the like, and the application of the single-stranded circular DNA in the field of biomedicine is effectively broadened. By means of the preparation method, a double-stranded assembly formed by complementation of the single-stranded circular DNA and fragmented single-stranded nucleic acid chains can be obtained, and the double-stranded assembly only needs to be subjected to an enzyme digestion reaction to obtain the single-stranded circular DNA, thereby effectively simplifying the synthesis steps of the existing single-stranded circular DNAs, and improving the synthesis efficiency; and the present invention is suitable for industrial large-