METHOD FOR PREPARING CIRCULAR SINGLE-STRAND RNA HAVING PRECISE SITE-DIRECTED MODIFICATION

Provided is a method for preparing a circular single-strand RNA. According to the method, by designing a first RNA fragment and a first DNA fragment, chemical modification of any precise site of a circular single-strand RNA can be achieved, so that the circular single-strand RNA has higher stability...

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Bibliographische Detailangaben
Hauptverfasser:	TIAN, Baolei, DONG, Yuanchen
Format:	Patent
Sprache:	chi ; eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Provided is a method for preparing a circular single-strand RNA. According to the method, by designing a first RNA fragment and a first DNA fragment, chemical modification of any precise site of a circular single-strand RNA can be achieved, so that the circular single-strand RNA has higher stability, a better binding capability, improved immunogenicity and the like, and a use of the circular single-strand RNA in the field of biomedicine is effectively broadened. By means of said preparation method, a double-strand assembly formed by complementation of a circular single-strand RNA and a fragmented single-strand nucleic acid chain can be obtained, and a circular single-strand RNA can be obtained simply by carrying out an enzyme digestion reaction on the double-strand assembly, so that the current synthesis steps of the circular single-strand RNA are effectively simplified, and the synthesis efficiency is improved. Thus, the preparation method is suitable for industrial large-scale preparation. L'invention conce