PRIMER COMPOSITION FOR DETECTING AVIAN INFLUENZA VIRUS AND NEWCASTLE DISEASE VIRUS ON BASIS OF MALDI-TOF MASS SPECTROMETRY DETECTION TECHNIQUE AND USE THEREOF

A primer composition for detecting avian influenza virus and newcastle disease virus on the basis of MALDI-TOF mass spectrometry detection technique and the use thereof. The primer composition comprises an amplification primer set as shown in SEQ ID Nos. 1-16 and a UEP extension primer set as shown...

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Hauptverfasser:	GAO, Zhiqiang, SONG, Yueqian, ZHONG, Yan, PU, Jing, GUO, Huimin, LAI, Pingan, GUO, Youran, ZHANG, Jiawei, ZHAO, Xiangpeng, WANG, Lin, REN, Tong, DU, Sile, ZHANG, Xiaohan, BAI, Zilong, ZHANG, Wei, SHI, Xiju
Format:	Patent
Sprache:	chi ; eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A primer composition for detecting avian influenza virus and newcastle disease virus on the basis of MALDI-TOF mass spectrometry detection technique and the use thereof. The primer composition comprises an amplification primer set as shown in SEQ ID Nos. 1-16 and a UEP extension primer set as shown in SEQ ID Nos. 17-31. The present invention further relates to a method for detecting avian influenza virus and newcastle disease virus on the basis of MALDI-TOF mass spectrometry detection technique. The MALDI-TOF mass spectrometry detection method is sensitive and specific, and can realize the synchronous detection of multiple targets. The method is not only suitable for the rapid detection and typing of main subtypes or strain types of avian influenza virus and newcastle disease virus in the throat/cloacal swab, tissue and organs in the import and export of poultry, and can also be used for rapid screening of main subtypes or strain types of avian influenza virus and newcastle disease virus in the samples from p