RECOMBINANT EXPRESSION VECTOR FOR PRODUCTION OF VIRUS-LIKE PARTICLE-BASED MULTIVALENT NOROVIRUS VACCINE AND MANUFACTURING METHOD THEREFOR

The present invention provides a fusion protein for promoting soluble expression of a norovirus antigen, and an expression vector thereof. More specifically, the present invention provides combinations of various genotypes of norovirus antigen proteins and RID mutants, in which the most efficient fo...

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Bibliographische Detailangaben
Hauptverfasser:	CHOI, Deog Young, JUNG, Hyun Gyo
Format:	Patent
Sprache:	eng ; fre ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides a fusion protein for promoting soluble expression of a norovirus antigen, and an expression vector thereof. More specifically, the present invention provides combinations of various genotypes of norovirus antigen proteins and RID mutants, in which the most efficient folding occurs when a norovirus antigen is used as a target protein to form a recombinant fusion protein, the yield of soluble expression of a fusion protein produced is improved, and the assembly efficacy and homogeneity of norovirus VLPs can be enhanced. Also, the present invention provides a method for rapidly mass-producing various other genotypes of norovirus VLPs, as well as GII.4, in E. coli by using mutant RID, thus enabling the development of a VLP-based multivalent vaccine comprising more genotypes. La présente invention concerne une protéine de fusion pour favoriser l'expression soluble d'un antigène de norovirus, et un vecteur d'expression de celle-ci. Plus particulièrement, la présente invention concerne des combinaisons de divers génotypes de protéines d'antigène de norovirus et de mutants de RID, dans lesquelles le pliage le plus efficace se produit lorsqu'un antigène de norovirus est utilisé en tant que protéine cible pour former une protéine de fusion recombinante, le rendement d'expression soluble d'une protéine de fusion produite est amélioré, et l'efficacité d'assemblage et l'homogénéité des VLP de norovirus peuvent être améliorées. La présente invention concerne également un procédé de production en masse rapide de divers autres génotypes de VLP de norovirus, ainsi que GII.4, dans E. coli à l'aide de RID mutant, permettant ainsi le développement d'un vaccin multivalent à base de VLP comprenant plus de génotypes. 본 발명은, 노로 바이러스 항원의 수용성 발현을 증진시키기 위한 융합단백질 및 이의 발현 벡터를 제공한다. 더욱 상세하게는, 노로 바이러스 항원을 목적 단백질로 하였을 때, 재조합 융합단백질 형성 시 가장 효율적으로 접힘이 일어나고, 제조되는 융합단백질의 수용성 발현 수율이 향상되며, 노로 바이러스 VLP의 조립 효늉과 균질성을 증진시킬 수 있는, 다양한 유전형의 노로바이러스 항원단백질 및 RID 돌연변이의 조합을 제공한다. 또한, 본 발명은 돌연변이 RID를 활용하여, GII.4 뿐만 아니라 다른 다양한 유전형의 노로바이러스 VLP를 대장균에서 대량으로 신속 생산할 수 있는 방법을 제공함으로써 보다 많은 유전형을 포함하는 VLP 기반의 다가 백신의 개발을 가능케 한다.