CARDIOMYOPATHY MODEL CELL LINE AND CARDIOMYOPATHY ORGANOID USING SAME

The present invention relates to a stem cell line with a modified LMNA gene and a cardiomyopathy cardiac organoid produced by using same, and in the present invention, a cell line modeling dilated cardiomyopathy was produced by modifying a specific nucleotide sequence of an LMNA gene sequence using...

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Bibliographische Detailangaben
Hauptverfasser:	NOH, Ji-Min, LIM, Do-Sun, HONG, Chae Yeon
Format:	Patent
Sprache:	eng ; fre ; kor
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CARE OF BIRDS, FISHES, INSECTS CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW BREEDS OF ANIMALS PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR SPIRITS TESTING TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a stem cell line with a modified LMNA gene and a cardiomyopathy cardiac organoid produced by using same, and in the present invention, a cell line modeling dilated cardiomyopathy was produced by modifying a specific nucleotide sequence of an LMNA gene sequence using gene editing technology, and by using same, a dilated cardiomyopathy cardiac organoid that well reflects characteristics of dilated cardiomyopathy was produced, and accordingly, the dilated cardiomyopathy heart organoid may be used for modeling of cardiomyopathy due to LMNA gene modification, and may be usefully employed in cardiomyopathy therapeutic agent screening. La présente invention concerne une lignée de cellules souches avec un gène LMNA modifié et un organoïde cardiaque de cardiomyopathie produit par son utilisation, et dans la présente invention, une lignée cellulaire modélisant la cardiomyopathie dilatée a été produite en modifiant une séquence nucléotidique spécifique d'une séquence du gène LMNA à l'aide d'une technique d'édition génique, et par son utilisation, il a été possible de produire un organoïde cardiaque de cardiomyopathie dilatée qui reflète bien les caractéristiques de la cardiomyopathie dilatée, et en conséquence, l'organoïde cardiaque de cardiomyopathie dilatée peut être utilisé pour la modélisation de la cardiomyopathie due à la modification du gène LMNA, et peut être utilisé utilement dans le criblage d'agents thérapeutiques destinés à lutter contre la cardiomyopathie. 본 발명은 LMNA 유전자가 변형된 줄기세포주 및 이를 이용하에 제작한 심근병증 심장 오가노이드에 관한 것으로, 본 발명에서는 LMNA 유전자 서열의 특정 염기 서열을 유전자 편집 기술로 변형함으로써 확장성 심근병증을 모델링한 세포주를 제작하였으며, 이를 이용하여 확장성 심근병증의 특성이 잘 반영된 확장성 심근병증 심장 오가노이드를 제작하였으므로, 이를 LMNA 유전자 변형에 기인한 심근병증의 모델링에 이용할 수 있으며, 심근병증 치료제 스크리닝에 유용하게 활용할 수 있다.