MIXED NANO-LIPID DELIVERY SYSTEM FOR MRNA, AND PREPARATION METHOD THEREFOR AND USE THEREOF

Disclosed are a mixed nano-lipid delivery system for mRNA, and a preparation method therefor and a use thereof. The delivery system is composed of a anionic and cationic mixed lipid, PEG2000-DSPE, a buffer system and mRNA; the anionic and cationic mixed lipid is composed of anionic nucleoside phospholipid TPS or CPS and cationic peptide lipid CLD or CLDA; and the buffer system is a PBS or Opti-MEMTM buffer system containing Ca2+ having a concentration 0.1 mM. The delivery system can efficiently deliver mRNA into cells and mice, and can realize long-time stable expression of proteins in vivo. In addition, an HPV E7 mRNA vaccine provided by the present invention can successfully activate humoral and cellular immunity in mice, reduce the mortality rate of HPV-related cervical cancer mice, and have good safety. In summary, the system has the advantages of good stability, high delivery efficiency, strong cell entry capacity, high biological activity and low toxicity, and will have a wide application prospect in th