AMPLICON BASED ROLLING CIRCLE AMPLIFICATION

The present invention concerns methods for detection of a target nucleic acid in a sample, comprising converting the target nucleic acid into an amplicon, for example using recombinase/polymerase amplification (RPA) or homologous recombination, further amplifying the amplicon by performing a rolling...

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Bibliographische Detailangaben
Hauptverfasser:	HURGIN, Vladimir, HANANI, Porat, VOLPERT, Giora, BEN EFRAIM, Alon Nissim, SHAYEVITCH, Ronna, DROMI, Ido, FARBEROV, Luba, ZAHAVY, Eran, CARSTENS MARQUES DE SOUSA, Keyla, SHULMAN, Anastasiya, KHWAJA, Aya
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention concerns methods for detection of a target nucleic acid in a sample, comprising converting the target nucleic acid into an amplicon, for example using recombinase/polymerase amplification (RPA) or homologous recombination, further amplifying the amplicon by performing a rolling circle amplification (RCA) and detecting the final RCA product. The invention further provides a cartridge, a kit and a system for performing the detection methods. La présente invention concerne des procédés de détection d'un acide nucléique cible dans un échantillon, comprenant la conversion de l'acide nucléique cible en un amplicon, par exemple à l'aide d'une amplification recombinase/polymérase (RPA) ou recombinaison homologue, l'amplification supplémentaire de l'amplicon par la mise en oeuvre d'une amplification en cercle roulant (RCA) et la détection du produit RCA final. L'invention concerne en outre une cartouche, un kit et un système pour mettre en oeuvre les procédés de détection.