PRODUCTION OF A DISACCHARIDE AND/OR MILK OLIGOSACCHARIDE BY A CELL WITH REDUCED SYNTHESIS OF UDP-GLCNAC

The present invention is in the technical field of synthetic biology, metabolic engineering and cell cultivation. The invention provides a cell for production of a disaccharide and/or an oligosaccharide like a milk oligosaccharide wherein synthesis of UDP-N-acetylglucosamine in said cell is rendered...

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Bibliographische Detailangaben
Hauptverfasser:	VERCAUTEREN, Annelies, DECOENE, Thomas, BEAUPREZ, Joeri
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention is in the technical field of synthetic biology, metabolic engineering and cell cultivation. The invention provides a cell for production of a disaccharide and/or an oligosaccharide like a milk oligosaccharide wherein synthesis of UDP-N-acetylglucosamine in said cell is rendered less functional. The invention further provides use of said cell in a cultivation or incubation. The invention also describes methods for the production of a disaccharide and/or an oligosaccharide like a milk oligosaccharide using said cell as well as the purification of said disaccharide and/or oligosaccharide like a milk oligosaccharide. La présente invention relève du domaine technique de la biologie synthétique, de l'ingénierie métabolique et de la culture cellulaire. L'invention concerne une cellule pour la production d'un disaccharide et/ou d'un oligosaccharide comme un oligosaccharide de lait, la synthèse de l'UDP-N-acétylglucosamine dans ladite cellule étant rendue moins fonctionnelle. L'invention concerne en outre l'utilisation de ladite cellule dans une culture ou une incubation. L'invention concerne également des procédés de production d'un disaccharide et/ou d'un oligosaccharide tel qu'un oligosaccharide de lait utilisant ladite cellule ainsi que la purification dudit disaccharide et/ou oligosaccharide comme un oligosaccharide de lait.