NUCLEIC ACID EXTRACTION VESSEL PROVIDED WITH POSITIVELY CHARGED POLYMER THIN FILM, AND SINGLE-POT NUCLEIC ACID DETECTION METHOD USING CHARGE TRANSFER POLYPLEX

A nucleic acid detection method comprises the steps of: inputting a sample containing nucleic acids into a vessel coated with a thin film comprising a cationic polymer having a hydrolyzable side chain; forming a polymer-nucleic acid complex by binding the nucleic acids to the cationic polymer which...

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Bibliographische Detailangaben
Hauptverfasser:	SONG, Younseong, LEE, Kyoung Gun, JO, Da Ae, SONG, Ja Yeon, KIM, Seongeun, PARK, Nahyun, KANG, Tae Joon, LIM, Sung Gap, JEONG, Booseok, PARK, Yu Min, JANG, Hyo Won
Format:	Patent
Sprache:	eng ; fre ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A nucleic acid detection method comprises the steps of: inputting a sample containing nucleic acids into a vessel coated with a thin film comprising a cationic polymer having a hydrolyzable side chain; forming a polymer-nucleic acid complex by binding the nucleic acids to the cationic polymer which has been dissolved from the thin film; releasing the nucleic acids from the polymer-nucleic acid complex by hydrolyzing the cationic polymer; and amplifying the nucleic acids or other nucleic acids synthesized from said nucleic acids. The nucleic acid detection method may be carried out in a single pot, may reduce the detection time, and may improve detection accuracy. Procédé de détection d'acides nucléiques comprenant les étapes suivantes : introduction d'un échantillon contenant des acides nucléiques dans un conteneur recouvert d'une couche mince comprenant un polymère cationique ayant une chaîne latérale hydrolysable ; constitution d'un complexe polymère-acide nucléique en liant les acides nucléiques au polymère cationique qui a été dissous de la couche mince ; libération des acides nucléiques du complexe polymère-acide nucléique par hydrolyse du polymère cationique ; et amplification des acides nucléiques ou d'autres acides nucléiques synthétisés à partir de ces acides nucléiques. Le procédé de détection des acides nucléiques peut être monotope, peut réduire le temps de détection et peut améliorer la précision de la détection. 핵산 검출 방법은, 가수분해 가능한 측쇄를 갖는 양이온성 중합체를 포함하는 박막이 코팅된 용기 내에 핵산을 포함하는 시료를 투입하는 단계, 상기 박막으로부터 용해된 양이온성 중합체와 상기 핵산을 결합시켜 중합체-핵산 복합체를 형성하는 단계, 상기 양이온성 중합체를 가수분해하여 상기 중합체-핵산 복합체로부터 상기 핵산을 방출하는 단계 및 상기 핵산 또는 상기 핵산으로부터 합성된 다른 핵산을 증폭하는 단계를 포함한다. 상기 핵산 검출 방법은 단일 포트에서 수행이 가능하며, 검출 시간을 단축시킬 수 있고, 검출 정확도를 향상시킬 수 있다.