EXPRESSION OF HUMAN PAPILLOMA VIRUS HPV39 L1 PROTEIN, VIRUS-LIKE PARTICLES, AND PREPARATION METHOD THEREFOR

An expression of the human papilloma virus HPV39 L1 protein, virus-like particles, and a preparation method therefor. The preparation method comprises: truncating the amino acid sequence of the HPV39 L1 protein, carrying out codon optimization on a coding nucleotide sequence of the truncated protein...

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Hauptverfasser:	CHEN, Xiao, YU, Hongyang, WANG, Xuehong, GAO, Wenshuang, WANG, Liying, SHEN, Ercui, LIU, Yuying, LIU, Yongjiang, WU, Shuming, YIN, Fei, ZHANG, Yao, XUE, Junlian, CHEN, Dan, ZHANG, Haijiang
Format:	Patent
Sprache:	chi ; eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Zusammenfassung:	An expression of the human papilloma virus HPV39 L1 protein, virus-like particles, and a preparation method therefor. The preparation method comprises: truncating the amino acid sequence of the HPV39 L1 protein, carrying out codon optimization on a coding nucleotide sequence of the truncated protein to obtain an optimized coding nucleotide sequence, and lastly matching with a tag-free expression vector having a specific SD sequence to achieve tag-free expression and purification. By means of the described improvement, higher protein expression is obtained in prokaryotic expression systems such as an escherichia coli expression system, and VLPs having more consistent quality are obtained. L'invention concerne une expression de la protéine L1 du papillomavirus humain HPV39, des particules pseudo-virales et un procédé de préparation associé. Le procédé de préparation comprend : la troncature de la séquence d'acides aminés de la protéine L1 de HPV39, la réalisation d'une optimisation de codon sur une séquence nuc