SPATIOTEMPORAL TRANSCRIPTOMIC SEQUENCING METHOD

Provided in the present disclosure is a spatiotemporal transcriptomic sequencing method, comprising: determining the cDNA abundance of a sample to be sequenced; and performing a spatiotemporal transcriptomic sequencing step on said sample on the basis of the cDNA abundance, wherein determining the c...

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Bibliographische Detailangaben
Hauptverfasser:	CHEN, Ao, XIANG, Haoxin, ZHAO, Yu, JIANG, Xia, LI, Zhaoxun, YUAN, Qiaomei, LIU, Weiqing, LIAO, Sha, ZHOU, Hui, ZHANG, Wenwei, CHEN, Dongdong
Format:	Patent
Sprache:	chi ; eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Provided in the present disclosure is a spatiotemporal transcriptomic sequencing method, comprising: determining the cDNA abundance of a sample to be sequenced; and performing a spatiotemporal transcriptomic sequencing step on said sample on the basis of the cDNA abundance, wherein determining the cDNA abundance of said sample comprising a pre-spatiotemporal transcriptomic sequencing step. According to the specific embodiments of the present disclosure, the cDNA abundance obtained by means of the method can accurately reflect the gene capture capability of the spatiotemporal transcriptomic technology on tissue slices. Compared with the prior art, the method omits steps of cDNA recovery and cDNA shearing, thereby greatly saving the operation time. La présente invention concerne un procédé de séquençage transcriptomique spatiotemporel, comprenant les étapes suivantes : établissement de l'abondance de l'ADNc d'un échantillon à séquencer ; et réalisation d'une étape de séquençage transcriptomique spacio-temporell