SPATIAL TRANSPOSITION-BASED RNA SEQUENCING LIBRARY PREPARATION METHOD

An RNA sequencing library preparation process that that utilized transposition with or with a template switch oligonucleotide to generate the libraries having UMIs and spatial barcode information, and methods for improving RNA library preparation from tissue samples using template switching and ther...

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Hauptverfasser:	PODDAR, Anustup, MATHER, Brian, GHAZINEJAD, Olivia, STORMS, Lena, FLOWERS, Brittany, OSTROW, Andrew, ZHANG, Kerou, MANZO, Andrea, CANON, Se, SHEN, Fei, EKSTRAND, Mats, XIAO, Yao, APRIL, Craig
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Zusammenfassung:	An RNA sequencing library preparation process that that utilized transposition with or with a template switch oligonucleotide to generate the libraries having UMIs and spatial barcode information, and methods for improving RNA library preparation from tissue samples using template switching and thermal amplification to improve RNA library quality. Un procédé de préparation de bibliothèque de séquençage d'ARN qui utilise une transposition avec ou sans oligonucléotide de commutation de modèle pour générer les bibliothèques ayant des UMI et des informations de code-barres spatial, et des procédés pour améliorer la préparation de bibliothèque d'ARN à partir d'échantillons de tissu à l'aide d'une commutation de modèle et d'une amplification thermique pour améliorer la qualité de bibliothèque d'ARN.