RECOMBINANT SACCHAROMYCES CEREVISIAE FOR PRODUCING CANNABIGEROLIC ACID, CONSTRUCTION METHOD THEREFOR, AND USE THEREOF

Provided is a recombinant Saccharomyces cerevisiae for producing cannabigerolic acid. The recombinant Saccharomyces cerevisiae is prepared from basic Saccharomyces cerevisiae, wherein in the basic Saccharomyces cerevisiae, the following endogenous genes are overexpressed, down-regulated, or knocked out: pep4, gal80, gal4, and fas1, and exogenous genes encoding the following enzymes are overexpressed: a tetraketone synthase (Cs.TKS) gene, an olivetolic acid cyclase (Cs.OAC) gene, a geranyl-pyrophosphate-olivetolic acid geranyltransferase (Cs.PT4n) gene, and a variant acetyl coenzyme synthase (Se.ACS) gene. The present application uses the Saccharomyces cerevisiae capable of synthesizing CBGA as an original strain, selectively overexpresses Cs.TKS, Cs.OAC, Cs.PT4, and Se.ACS, selectively knocks out gal80 and overexpresses gal4, selectively knocks out pep4, selectively overexpresses Cs.OAC fused with or without a linker, and positions the Cs.PT4n enzyme to the plasma membrane with or without a signal peptide, th