METHOD FOR ISOLATING PLANT-DERIVED EXOSOME, EXOSOME ISOLATED THEREBY, AND COSMETIC USE THEREOF

The present disclosure provides a method for isolating an exosome with increased exosome isolation efficiency, and a cosmetic composition comprising an exosome having improved wrinkle amelioration, cell proliferation, wound healing, or whitening effects. Disclosed is a method for extracting a plant-...

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Bibliographische Detailangaben
Hauptverfasser:	JEON, Ju Hee, PARK, Sun Young, JUNG, Sung Won, JUNG, Hyo Il, CHO, Se Hee, KIM, Yoon Ah, CHO, Youn Ki
Format:	Patent
Sprache:	eng ; fre ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present disclosure provides a method for isolating an exosome with increased exosome isolation efficiency, and a cosmetic composition comprising an exosome having improved wrinkle amelioration, cell proliferation, wound healing, or whitening effects. Disclosed is a method for extracting a plant-derived exosome, comprising: (a) a step of washing and juicing a plant to obtain a plant juice liquid; (b) a primary centrifugation step of obtaining a primary supernatant by centrifuging the plant juice liquid; (c) a secondary centrifugation step of obtaining a second supernatant by centrifuging the primary supernatant; (d) subjecting the second supernatant to tangential flow filtration (TFF) to obtain an isolate; and (e) adding an antibody-attached bead to the isolate to obtain an extract including a plant-derived exosome. Also disclosed is a cosmetic composition comprising a plant-derived exosome in an amount of 0.1-20 wt% of the total weight of the composition. La présente invention propose un procédé d'isolement d'un exosome avec une efficacité d'isolation d'exosome accrue, et une composition cosmétique comprenant un exosome présentant une atténuation améliorée des rides, une prolifération cellulaire, une cicatrisation des plaies, ou des effets de blanchiment de la peau. La présente invention concerne un procédé d'extraction d'un exosome issu d'une plante, comprenant : (a) une étape de lavage et de réduction en jus d'une plante pour obtenir un jus de plante liquide ; (b) une étape de centrifugation primaire pour obtenir un surnageant primaire par centrifugation du jus de plante liquide ; (c) une étape de centrifugation secondaire pour obtenir un deuxième surnageant par centrifugation du surnageant primaire ; (d) soumission du deuxième surnageant à une filtration à flux tangentiel pour obtenir un isolat ; et (e) ajout d'une bille fixée sur un anticorps à l'isolat pour obtenir un extrait comprenant un exosome issu de la plante. La présente invention concerne également une composition cosmétique comprenant un exosome issu de plantes dans une proportion de 0,1 à 20 % en poids du poids total de la composition. 본 개시는 엑소좀의 분리 효율이 증대된 엑소좀 분리 방법과, 주름개선, 세포 증식, 상처 치유 또는 미백 효과가 향상된 엑소좀을 포함하는 화장료 조성물을 제공하고자 한다. 본 개시는 (a) 식물을 세척 및 착즙하여 식물 착즙액을 수득하는 단계; (b) 식물 착즙액을 원심분리하여 1차 상층액을 수득하는 1차 원심분리 단계; (c) 1차 상층액을 원심분리하여 2차 상층액을 수득하는 2차 원심분리 단계; (d) 2차 상층액을 접선유동여과(tangential flow filtration, TFF)하여 분리물을 수득하는 단계; 및 (e) 분리물에 항체가 부착된 비드를 첨가하여 식물 유래 엑소좀을 포함하는 추출물을 얻는 단계;를 포함하는 식물