PBASE PROTEIN, FUSION PROTEIN, NUCLEIC ACID, GENE INTEGRATION SYSTEM AND USE

Provided in the present invention are a PBase protein, a fusion protein, a nucleic acid, a gene integration system, a gene delivery system and the use. The PBase protein retains the abilities of binding to the ITR sequences of a Piggybac transposon and cleaving the Piggybac transposon to make the Piggybac transposon separate from a donor plasmid in a linear manner; and the PBase protein has a lower ability for binding to the double strands of DNA within 10 bp upstream and downstream of "TTAA". When the PBase protein is linked to a site-specific DNA nuclease to prepare the fusion protein, the PBase protein can be efficiently integrated with a long fragment gene of interest at the double strand breaks generated by the site-specific DNA nuclease, thereby achieving the effect of site-directed insertion of the long fragment gene. La présente invention concerne une protéine PBase, une protéine de fusion, un acide nucléique, un système d'intégration de gène, un système de délivrance de gène et l'utilisation. La prot